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制备抗羊布鲁菌核糖体蛋白L7/L12的单克隆抗体(mAb),为进一步研制布鲁菌检测方法和免疫制剂提供基础。常规动物免疫、细胞融合、克隆化制备抗核糖体蛋白L7/L12的mAb,并用Westernblot和ELISA方法对其特异性、抗原识别表位及相对亲和力等做了初步鉴定。获得了4株抗L7/L12蛋白的mAb:1B1,2A2,2H9和2H10,相对亲和力为2A2>2H10>2H9>1B1,4株mAb识别的抗原表位相近,但仍存在一定的差异。得到的4株稳定的杂交瘤细胞系分泌的mAb能特异结合L7/L12蛋白。
Preparation of monoclonal antibodies (mAb) against Brucella abortus ribosomal protein L7 / L12 provided the basis for further development of Brucella detection methods and immunological preparations. Conventional animal immunization, cell fusion and cloning were used to prepare anti-ribosomal protein L7 / L12 mAb. The specificities, epitopes and relative affinities of antigens were preliminarily identified by Western blot and ELISA. Four anti-L7 / L12 mAbs: 1B1, 2A2, 2H9 and 2H10 were obtained, and the relative epitopes of 2A2> 2H10> 2H9> 1B1 and 4 mAbs were similar, but there were still some differences. The mAbs secreted by the four stable hybridoma cell lines obtained could specifically bind to the L7 / L12 protein.