基于可逆饱和光转移过程的荧光超分辨显微技术,从原理上打破了原有的光学远场衍射极限对光学系统极限分辨率的限制,在生物、化学、医学等多个学科拥有广泛的应用前景。回顾了近年来超分辨显微研究的历史,综述了目前常见的几种基于可逆饱和光转移过程的荧光超分辨显微方法,详细描述了各自的技术特点并对比了其优缺点,阐述了相关领域内最新的研究工作进展。 The fluorescence super-resolution microscopy based on reversible saturated light transfer process breaks the limitations of the original optical far-field diffraction limit on the limit resolution of optical system in principle, and has a wide range of applications in many disciplines such as biology, chemistry and medicine prospect. The history of super-resolution microscopy in recent years is reviewed. Several commonly used fluorescence super-resolution microscopic methods based on reversible saturated light transfer are reviewed. Their respective technical features are described in detail and their advantages and disadvantages are compared. Relevant correlations The latest developments in the field of research.