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目的:建立高效液相色谱法测定氯甲霜中氯霉素和甲硝唑含量。方法:色谱柱Diamonsil C_(18)(250 mm×4.6 mm,5μm),流动相为0.1%庚烷磺酸钠溶液(0.1%庚烷磺酸钠溶液500 ml与二甲基甲酰胺5 ml,冰醋酸0.5 ml)-乙腈(80:20),流速为1.0 ml·min~(-1),检测波长为278 nm。结果:氯霉素和甲硝唑浓度在30～150μg·ml~(-1)范围内均呈良好的线性关系,r=0.999 6和r=0.998 1;平均回收率分别为101.23%(RSD=0.69%),99.63%(RSD=0.61%)(n=9)。结论:本方法简便,准确,可用于氯甲霜中氯霉素和甲硝唑的含量测定。
Objective: To establish a HPLC method for the determination of chloramphenicol and metronidazole in chlormethylation cream. METHODS: Diamonsil C 18 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of 0.1% sodium heptanesulfonate solution (0.1% sodium heptane sulfonate solution and 5 ml dimethylformamide solution) Glacial acetic acid 0.5 ml) - acetonitrile (80:20) at a flow rate of 1.0 ml · min ~ (-1) at a detection wavelength of 278 nm. Results: The linear range of chloramphenicol and metronidazole was 30 ~ 150μg · ml ~ (-1), r = 0.999 6 and r = 0.998 1, respectively. The average recoveries were 101.23% (RSD = 0.69%), 99.63% (RSD = 0.61%) (n = 9). Conclusion: The method is simple and accurate and can be used for the determination of chloramphenicol and metronidazole in chlormethylation cream.