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肿瘤坏死因子(TNF)在多种病因导致急性肺损伤(ALI)或ARDS的进程中发挥着重要作用,但在急性坏死性胰腺炎(ANP)时肺内TNF-α基因的过度表达与并发肺损伤的关系尚未获确切证据。我们拟从分子水平探讨急性胰腺炎时肺内TNF-α基因转录与蛋白表达状况在肺损伤发病机制中的意义。 1 材料和方法 SD大鼠90只随机分为A,B,C三组,A组以5g/L牛磺胆酸钠胰胆管内逆行注射制成急性水肿性胰腺炎(AEP)模型,B组同法以50g/L牛磺胆酸钠制成ANP模型,C组为假手术对照组。各组动物分别于预定时点处死,测定血淀粉酶、内毒素(ET)及TNF-α水平;以半定量逆转录聚合酶链反应(RT-PCR)技术检测肺组织中TNF-α mRNA含量了解基因转录水平,以免
Tumor necrosis factor (TNF) plays an important role in the pathogenesis of acute lung injury (ALI) or ARDS in a variety of etiologies. However, TNF-alpha gene overexpression and pulmonary involvement in acute necrotizing pancreatitis (ANP) The exact relationship between injuries has yet to be confirmed. We intend to explore the molecular level of lung TNF-α gene transcription and protein expression in the pathogenesis of lung injury. 1 Materials and Methods 90 SD rats were randomly divided into A, B, C three groups, A group of 5g / L sodium taurocholate pancreaticoduodenea retrograde injection of acute edematous pancreatitis (AEP) model, B group The same method to 50g / L sodium taurocholate ANP model, C group sham operation control group. The animals were sacrificed at predetermined time points, and the levels of serum amylase, endotoxin (ET) and TNF-α were determined. The content of TNF-α mRNA in lung tissue was detected by semi-quantitative reverse transcription polymerase chain reaction Understand the level of gene transcription, lest