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目的 研究13N-氨水(13N-NH3·H2O)的临床前药理,探讨13N-NH3·H2O的PET显像方法及其在心脏疾患诊断中的应用。方法 测定13N-NH3·H2O在小鼠体内的分布,并利用其进行犬的全身和动态心肌PET显像,测定各器官对13N-NH3·H2O的摄取及其动态分布。 结果 小鼠心肌对13N-NH3·H2O的摄取率最高,占25.3%。犬动态PET显像发现,注射13N-NH3·H2O后30秒,右心室和左心室血池的放射性达到峰值,1 min后明显下降;左心室间壁和侧壁心肌在第20秒开始摄取,间壁摄取高于侧壁,1 min后侧壁摄取高于间壁,侧壁与间壁的放射性活度比值为1.20±0.55;5 min后心/血、心/肝和心/肺比值高于2.5。犬全身PET显像发现,13N-NH3·H2O主要分布在血供丰富的组织,肾脏是主要的清除器官。 结论 13N-NH3·H2O是理想的心肌血流灌注显像剂,可以无创地精确评估局部心肌血流灌注。
Objective To study the preclinical pharmacology of 13N-NH3 (13N-NH3 · H2O) and explore the PET imaging method of 13N-NH3 · H2O and its application in the diagnosis of heart disease. Methods The distribution of 13N-NH3 · H2O in mice was determined and the whole body and dynamic myocardial PET imaging of dogs were performed. The uptake and dynamic distribution of 13N-NH3 · H2O in various organs were determined. Results The uptake rate of 13N-NH3 · H2O in mouse myocardium was the highest, accounting for 25.3%. Dynamic PET imaging in dogs showed that radioactivity in the right ventricle and left ventricular blood pool peaked at 30 seconds after injection of 13N-NH 3 · H 2 O, and decreased significantly after 1 min. The left ventricular and lateral myocardium began to be ingested at 20 seconds, The ratio of radioactivity of side wall uptake to wall, side wall and side wall was 1.20 ± 0.55 after 1 minute after ingestion. The ratio of heart / blood, heart / liver and heart / lung was higher than 2.5 after 5 minutes. PET whole body image analysis found that dogs, 13N-NH3 · H2O mainly in blood-rich tissue, the kidneys are the main removal organs. Conclusion 13N-NH3 · H2O is an ideal myocardial perfusion imaging agent, which can accurately assess the local myocardial perfusion.