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复制大鼠免疫性肝纤维化模型 ,采用Griess反应法测定一氧化氮 (NO) ,结晶紫染色法测定肿瘤坏死因子α(TNF α) ,胸腺细胞法测定白介素 1(IL 1) ,并用逆转录聚合酶链反应 (RT PCR)法检测mRNA的表达。结果 :(1)软肝化丸各剂量组均能明显抑制血清丙氨酸氨基转移酶 (ALT)、门冬氨酸氨基转移酶 (AST)的升高 ,提高白蛋白 (A1b) ,减轻肝细胞坏死、变性及胶原增生程度 ;(2 )抑制大鼠腹腔巨噬细胞产生NO、TNF α、IL 1;(3)抑制大鼠肝组织Ⅰ胶原、Ⅲ型前胶原mRNA的表达。
Rat model of immune hepatic fibrosis was replicated, nitric oxide (NO) was measured by Griess reaction, tumor necrosis factor alpha (TNF α) was measured by crystal violet staining, interleukin 1 (IL 1) was measured by thymocyte method, and reverse transcription was performed. The expression of mRNA was detected by polymerase chain reaction (RT PCR). Results: (1) Ruangan Huayu Pills can significantly inhibit the elevation of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and increase albumin (A1b) in each dose group. Hepatocyte necrosis, degeneration and collagen hyperplasia; (2) Inhibition of rat peritoneal macrophage production of NO, TNFα, IL 1; (3) inhibition of rat liver collagen I, III procollagen mRNA expression.