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目的探讨磺草酮(一种三酮类除草剂)亚慢性暴露对大鼠糖代谢和酪氨酸代谢的影响。方法以不同剂量磺草酮(5、505、00 mg/(kg.d))混饲染毒90 d。分别测定第零天、306、0、90 d空腹血糖;实验结束后行糖耐量试验;并检测肝脏4-羟苯丙酮酸二加氧酶(HPPD)活力及血清酪氨酸、肾上腺素含量。结果染毒组大鼠各时间点空腹血糖,糖耐量及血清肾上腺素水平与对照组相比,其差异均无统计学意义;染毒组大鼠肝脏HPPD活力与对照组相比均明显下降(P<0.001);染毒组大鼠血清酪氨酸水平与对照组相比均明显升高(P<0.001),且酪氨酸水平有随染毒剂量增高而升高的趋势。结论磺草酮有效抑制大鼠肝脏HPPD活力,干扰大鼠酪氨酸代谢,诱发严重酪氨酸血症。尽管酪氨酸还是肾上腺素等儿茶酚胺类激素的前提物质,但在本实验中未发现大鼠肾上腺素发生显著性改变,同时也未见糖代谢异常。
Objective To investigate the effects of sulcotrione (a triketone herbicide) sub-chronic exposure on glucose metabolism and tyrosine metabolism in rats. Methods Different doses of sulcotrione (5,505,00 mg / (kg · d)) were mixed for 90 days. The fasting blood glucose was measured at day 0, day 306, day 30, day 90; the glucose tolerance test was performed at the end of the experiment; and the hepatic 4-hydroxyphenylpyruvatedioxygenase (HPPD) activity, serum tyrosine and epinephrine contents were measured. Results Compared with the control group, the fasting blood glucose, glucose tolerance and serum adrenaline levels were not significantly different at each time point in the exposure group. The hepatic HPPD activity in the exposed group was significantly lower than that in the control group P <0.001). The level of tyrosine in the exposed group was significantly higher than that in the control group (P <0.001), and the level of tyrosine tended to increase with the increase of the dose. Conclusion sulcotrione effectively inhibited rat liver HPPD activity, interfering with tyrosine metabolism in rats and inducing severe tyrosinemia. Although tyrosine or adrenaline and other catecholamines, the premise of substances, but in this experiment did not find significant changes in adrenergic rats, but also did not see abnormal glucose metabolism.