为研究n-3脂肪酸对内皮的保护功能,以内皮通透性作指标,采用伊文氏蓝为示踪剂,使用荧光显微镜研究通透深度,使用丙酮-生理盐水抽提分光光度计测定通透量。结果表明高胆固醇血症大鼠模型内皮通透性明显增加(P<0.001),n-3脂肪酸可显著拮抗通透性增加(P<0.01)。说明n-3脂肪酸对动脉粥样硬化的有益作用机制之一是保护内皮功能。 In order to study the protective function of n-3 fatty acids on endothelial cells, endothelial permeability was used as an index, Evans blue as a tracer, the depth of penetration was studied by fluorescence microscopy, and the permeability was measured by acetone-saline extraction spectrophotometer the amount. The results showed that hypercholesterolemic rats model endothelial permeability increased significantly (P <0.001), n-3 fatty acids can significantly antagonize the increased permeability (P <0.01). Description of n-3 fatty acid on atherosclerosis beneficial mechanism of action is to protect the endothelial function.