论文部分内容阅读
该文检测了在急性缺血性脑卒中(acute ischemic stroke,AIS)患者血清中微RNA32-3p(mi R-32-3p)水平,探讨了其与AIS发病的相关性。采用实时荧光定量聚合酶链反应法(RTq PCR)测定89例AIS患者及89例健康对照者血清mi R-32-3p水平。运用生物学数据库预测mi R-32-3p的靶基因,采用DAVID数据库对靶基因集合进行功能注释分析。结果显示,与对照组相比较,AIS组血清mi R-32-3p水平升高约3.28倍,差异极显著性(P<0.001)。mi R-32-3p可能参与缺血性卒中的脑缺血性损伤、再灌注损伤和脑缺血后细胞凋亡。结果表明,血清mi R-32-3p水平升高与AIS有密切相关性,其可能为AIS的潜在生物标志物之一。
This study examined the serum levels of microRNA32-3p (mi R-32-3p) in patients with acute ischemic stroke (AIS) and explored its association with the pathogenesis of AIS. Serum mi R-32-3p levels were measured in 89 patients with AIS and 89 healthy controls by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The biological database was used to predict the mi R-32-3p target gene, and the DAVID database was used to perform functional annotation analysis on the target gene pool. The results showed that compared with the control group, the serum mi R-32-3p level in AIS group increased by about 3.28-fold, the difference was significant (P <0.001). mi R-32-3p may be involved in ischemic cerebral ischemic injury, reperfusion injury and apoptosis after cerebral ischemia. The results show that serum mi R-32-3p levels are closely related with AIS, which may be one of the potential biomarkers of AIS.