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为测定糖皮质激素(GCS)对表皮郎格罕细胞(LC)的直接作用,作者在试管内用地塞米松(DEX)处理分离的鼠表皮LC,并用免疫荧光显微镜检查和荧光激活细胞分离仪(FACS)分析研究LC的多种抗原标记(例如Ia)的表达。作者发现:(1)当用免疫荧光显微镜检查时,醋酸肤轻松溶液外用于鼠耳,2次/d,共4天后,发现鼠局部皮肤的I-A抗原、Ia恒定链和NLDC145抗原阳性的LC均显著减少。从鼠耳和躯干部取皮制备表皮细胞(EC)悬液,分别与不同浓度
To determine the direct effect of glucocorticoid (GCS) on epidermal Langerhans cells (LC), the authors treated the isolated mouse epidermal LC with dexamethasone (DEX) in vitro and immunofluorescence microscopy and fluorescence activated cell sorter FACS) analysis of the expression of multiple antigenic markers of LC (eg, Ia). The authors found that: (1) When immunofluorescence microscopy was used, skin lightening solution of acetate was applied to rat ears twice a day for 4 days, and it was found that the LC antigen of the local skin of mice, Ia constant strand, and LC positive for NLDC145 antigen Significantly reduced. Epidermal cells (EC) suspensions were prepared from the ears and trunk of rats, respectively, at different concentrations