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目的观察反复缺血再灌注致血管性痴呆(Vascular dementia,以下简称VD)大鼠血清基质金属蛋白酶-9(MMP-9)、血浆血栓素B2(TXB2)、6-酮-前列腺素(6-Keto-PGF1a)变化及通络中药的影响,探讨通络中药对急性脑损伤的干预机制。方法实验采用反复夹闭双侧颈总动脉,同时腹腔注射硝普钠的方法复制血管性痴呆大鼠模型。于灌胃干预2周后放射免疫法检测大鼠血浆中TXB2、6-Keto-PGF1a含量,采用双抗体夹心ABC-ELISA法测定血清MMP-9表达。结果①通络中药可使血管性痴呆大鼠血浆中TXB2表达显著减少,6-Keto-PGF1a含量明显升高,T/P比值下调;②各组大鼠血清MMP-9表达未见明显差异。结论通络中药可通过降低TXB2/6-Keto-PGF1a比值纠正二者之间的平衡失调,改善脑部供血,发挥脑保护作用。缺血再灌注后两周模型组大鼠血清MMP-9表达呈现正常水平,其表达高峰可能已经结束。
Objective To observe the serum levels of matrix metalloproteinase-9 (MMP-9), plasma thromboxane B2 (TXB2), and 6-keto-prostaglandin (6-keto-prostaglandin) in rats with vascular dementia (VD) induced by repeated ischemia reperfusion (6) Keto-PGF1a) changes and influence of Tongluo Chinese medicine, to explore the intervention mechanism of Tongluo Chinese medicine on acute brain injury. METHODS: A rat model of vascular dementia was replicated by repeatedly clipping the bilateral common carotid artery and intraperitoneally injecting sodium nitroprusside. Radioimmunoassay was used to detect the content of TXB2,6-Keto-PGF1a in rat plasma after 2 weeks of intragastric administration. The serum MMP-9 was detected by double antibody sandwich ABC-ELISA. Results 1 The Tongluo Chinese medicine can significantly reduce the TXB2 expression in plasma of vascular dementia rats, and the content of 6-Keto-PGF1a is significantly increased, and the T/P ratio is decreased. 2 There is no significant difference in the expression of serum MMP-9 in each group. Conclusion Tongluo traditional Chinese medicine can correct the imbalance between the two by reducing the ratio of TXB2/6-Keto-PGF1a, improve brain blood supply, and exert brain protection. Two weeks after ischemia-reperfusion, the expression of MMP-9 in the serum of the model group showed a normal level, and the peak of expression may be over.