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目的 :探讨MHCI类分子限制性肿瘤抗原多肽Mutl体外冲击白细胞介素 2 (Interleukin 2 ,IL 2 )基因修饰的树突状细胞 (Dendrticcells,DC)对小鼠体内特异性免疫的活化机制。方法 :用腺病毒作为载体介导小鼠IL 2基因修饰DC ,用小鼠Lewis肺癌 3LL细胞株MHCI类分子限制性八肽Mutl冲击IL 2基因修饰的DC(DC IL 2 Mutl)免疫小鼠 ,用流式细胞术 (FACS)分析免疫保护小鼠接受 3LL细胞再攻击后引流淋巴结内T细胞亚群的比例变化。结果 :用Mutl冲击的DC免疫的小鼠抵抗3LL细胞再攻击时 ,引流淋巴结内CD8+ T淋巴细胞比例明显升高 ,DC IL 2 Mutl免疫保护的小鼠接受 3LL细胞再攻击后引流淋巴结内CD8+ 和NK细胞的比例都明显升高。结论 :研究表明MHCI类分子限制性肿瘤抗原多肽体外冲击IL 2基因修饰的DC免疫小鼠 ,能诱导小鼠产生特异性抗肿瘤免疫应答 ,并能活化小鼠体内多环节免疫功能
OBJECTIVE: To investigate the mechanism of activation of specific immunity induced by MHC class I tumor suppressor peptide Mutl in vitro by dendrtic cells (DCs) modified by Interleukin 2 (IL 2) gene. Methods: Mouse DC2 gene was modified by adenovirus vector to induce DCs. Mice were immunized with IL2 gene modified DCs (DC IL2 Mut1) Flow cytometry (FACS) was used to analyze the proportion of T lymphocyte subsets in the draining lymph nodes after immunization in 3LL cells. Results: The mice immunized with Mutl against DCs challenged with 3LL cells were further challenged. The proportion of CD8 + T lymphocytes in draining lymph nodes was significantly increased. DC IL 2 Mut1 immunized mice received 3LL cells and then drained CD8 + and NK cells were significantly increased. CONCLUSION: The study shows that MHC class I-restricted tumor antigen peptides impact IL 2 gene-modified DC immunized mice in vitro can induce mice to produce specific anti-tumor immune response and activate multi-link immune function in mice