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采用饲养试验方法,研究了平均体质量为(7.16±0.07)g的大菱鲆(Scophthalmus maximus)幼鱼分别在盐度12、18、24、30和36下饲养60 d后,其鳃Na+-K+-ATPase活力、血清离子浓度、生长激素(GH)、皮质醇激素(COR)、特定生长率(SGR)和饲料效率(FCE)的变化。结果表明:幼鱼鳃Na+-K+-ATPase活力、血清Na+浓度均随盐度的升高而上升,分别在3.48~8.30 U/mg和169.99~180.00 mmol/L之间,其中12盐度组最低,36盐度组最高。幼鱼血清中K+和Cl-浓度分别在2.20~3.47 mmol/L和136.67~142.00 mmol/L之间,各盐度组之间差异不显著。幼鱼血清中GH和COR浓度分别在0.41~1.66 ng/ml和35.33~76.41 ng/ml之间;其中GH在36盐度组最高,12盐度组最低,而COR在12盐度组最高,36盐度组最低。幼鱼SGR和FCE分别在(1.45~2.00)%/d和1.12%~1.38%之间,与盐度的相关性不显著,两者均为12盐度组最低。由此可见,盐度变化显著影响大菱鲆幼鱼鳃Na+-K+-ATPase活力、血清Na+浓度和激素含量。本研究对大菱鲆养殖生理生态条件分析具有重要参考意义,研究结果可为大菱鲆养殖的盐度选择提供理论依据。
Using feeding experiment, the juvenile Scophthalmus maximus (7.16 ± 0.07 g) body weight was fed with salinity of 12, 18, 24, 30 and 36 respectively for 60 d, and its gill Na + K + -ATPase activity, serum ion concentration, GH, COR, SGR and FCE. The results showed that Na + -K + -ATPase activity and serum Na + concentration of gills increased with salinity increasing, ranging from 3.48 to 8.30 U / mg and 169.99 to 180.00 mmol / L, respectively, with the lowest in 12-salinity group , 36 salinity group highest. The concentrations of K + and Cl- in serum of juvenile fish were between 2.20 and 3.47 mmol / L and between 136.67 and 142.00 mmol / L, respectively. There were no significant differences among the salinity groups. The concentrations of GH and COR in juvenile fish serum ranged from 0.41 to 1.66 ng / ml and from 35.33 to 76.41 ng / ml, respectively. Among them, GH was the highest in 36 salinity and lowest in 12 salinity, while COR was the highest in 12 salinity, 36 salinity group lowest. The juvenile SGR and FCE were between 1.45 and 2.12% and 1.12% and 1.12% and 1.38%, respectively. The correlation between salinity and SGR was insignificant, both of which were the lowest among 12 salinity groups. Thus, changes in salinity significantly affect gill Na + -K + -ATPase activity, serum Na + concentration and hormone content in juvenile turbot. This study has important reference significance for the analysis of the physiological and ecological conditions of turbot culture, and the results can provide the theoretical basis for salinity selection of turbot culture.