论文部分内容阅读
肝细胞性肝癌（ＨＣＣ）与ＨＢＶ整合密切相关，为研究ＨＢＶ－ＤＮＡ各片段在ＨＣＣ发生中的作用，用ＨＢＶ全长基因探针通过Ｓｏｕｔｈｅｒｎ转膜杂交分析了１７例ＨＣＣ标本中ＨＢＶ－ＤＮＡ的存在状态，并用ＨＢＶ片段基因探针和Ｎ－ｒａｓ、ｐ５３探针检测了其相应ｍＲＮＡ的转录情况。结果发现，５２．９％ＨＣＣ标本为纯整合型，４７．１％的标本为混合型。１７例ＨＣＣ中，Ｐｒｅｓ、ＨＢｓ、ＨＢｘ、ＨＢｃ转录率分别为４７．１％、５８．８％、７０．５％和４１．２％。９例纯整合型ＨＣＣ中，上述基因转录率分别为１１．１％、４４．４％、５５．６％和１１．１％，其中ＨＢｘ较ＨＢｃ、Ｐｒｅｓ差异有显著性（Ｐ＜０．０５），ＨＣＣ标本Ｎ－ｒａｓ、ｐ５３总转录率分别为８２．３％、６４．７％。提示ＨＢＶ－ＤＮＡ在ＨＣＣ细胞内主要以整合于染色体上的方式存在，而且整合片段可作为转录的模板，其蛋白产物可能通过影响Ｎ－ｒａｓ、ｐ５３在细胞调控中的功能促进肿瘤的形成。
Hepatocellular carcinoma (HCC) is closely related to the integration of HBV. To study the role of HBV-DNA fragments in the development of HCC, HBV-DNA was analyzed in 17 cases of HCC specimens by Southern transmbrane hybridization using HBV full-length gene probes. Existence status, and using HBV fragment gene probes and N-ras, p53 probe to detect the corresponding mRNA transcription. The results showed that 52.9% of HCC samples were purely integrated, and 47.1% of samples were mixed. In 17 cases of HCC, the transcription rates of Pres, HBs, HBx, and HBc were 47.1%, 58.8%, 70.5%, and 41.2%, respectively. In 9 cases of purely integrated HCC, the above gene transcription rates were 11.1%, 44.4%, 55.6%, and 11.1%, respectively, among which HBx was significantly different from HBc and Pres (P<0.05). The total transcription rates of N-ras and p53 in HCC specimens were 82.3% and 64.7%, respectively. It is suggested that HBV-DNA is mainly integrated into chromosomes in HCC cells, and the integration fragment can be used as a template for transcription. Its protein products may promote the formation of tumors by affecting the functions of N-ras and p53 in cell regulation.