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采用微粒吸附法从广东省一慢性丙型肝炎病人血清中提取丙型肝炎病毒（ＨＣＶ）ＲＮＡ，经随机引物逆转录为ｃＤＮＡ，再经聚合酶链反应（ＰＣＲ），获得包膜蛋白区（Ｅ１，Ｅ２／ＮＳ１）两个部分重叠的产物片段，分别为４８９ｂｐ和８０６ｂｐ。其中，４８９ｂｐ片段主要位于Ｅ１区，小部分位于Ｃ区；８０６ｂｐ片段位于Ｅ２／ＮＳ１区。将４８９ｂｐ片段插入ｐＵＣ１９载体，再亚克隆进ｐＵＣ１８质粒载体。８０６ｂｐ片段则插入到ｐＢＶ２２０质粒及Ｍ１３ｍｐ１８、Ｍ１３ｍｐ１９噬菌体。将所测定的序列（共为１２４６ｂｐ）与多个已知分离株相应序列比较后发现，核苷酸同源性介乎６１．００％－８９．４１％，与Ⅱ（１ｂ）型同源性最大，超过８６％；推导的氨基酸同源性为６０．２４％－８７．２３％。与其它株比较，ＨＣＶ广东株（ＨＣＶ－ＧＤ）较大变异区包括２５１－２５８、３８３－４０６、４３４－４４６及４７５－４８０位氨基酸。其中两个与公认的高变异区（３８３－４０８、４７５－４８０）相对应。
Hepatitis C virus (HCV) RNA was extracted from the serum of a chronic hepatitis C patient in Guangdong province by microparticle adsorption. The cDNA was reverse transcribed into cDNA by random primers and then amplified by polymerase chain reaction (PCR) , E2 / NS1) two partially overlapping product fragments, 489 bp and 806 bp, respectively. Among them, the 489bp fragment is mainly located in the E1 region, a small part in the C region; 806bp fragment in the E2 / NS1 region. The 489 bp fragment was inserted into pUC19 vector and subcloned into pUC18 plasmid vector. The 806 bp fragment was inserted into pBV220 plasmid and M13mp18, M13mp19 phage. Comparison of the determined sequences (1246bp in total) with the corresponding sequences of several known isolates revealed nucleotide homologies ranging from 61.00% to 89.41%, with type II (1b) homology The largest, more than 86%; deduced amino acid homology of 60.24% -87.23%. Compared with other strains, the larger variation region of HCV Guangdong strain (HCV-GD) includes 251-258, 383-406, 434-446 and 475-480 amino acids. Two of these correspond to the accepted hypervariable regions (383-408, 475-480).