免疫组化技术在病理诊断、基础医学研究工作中已成为非常重要的手段。随着免疫组化试剂出现及方法的改进,特别是即用型试剂盒的出现,使抗体的标记更加简便、快捷、规范化。但是由于它是一个多步骤、多因素决定的一种实验方法,由于各实验室采用的修复方法、染色方法、试剂厂家、抗体克隆号、检测系统等有所不同,染色结果会出现较大差异。建立标准化的染色程序,改善和提高免疫组化实验的可靠性,使免疫组化技术更具标准化,免疫 Immunohistochemistry in pathological diagnosis, basic medical research has become a very important tool. With the emergence of immunohistochemical reagents and the improvement of methods, especially the emergence of ready-to-use kits, the antibody labeling is easier, faster and more standardized. However, since it is a multi-step and multi-factorial experimental method, the staining results will be greatly different due to the different repair methods, staining methods, reagent manufacturers, antibody clone numbers and detection systems adopted by various laboratories . Establishing a standardized staining program to improve and enhance the reliability of immunohistochemical experiments, to standardize immunohistochemical techniques and to immunize