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目的探讨四联活菌(保加利亚杆菌、幼儿双歧杆菌、芽孢杆菌和嗜酸乳杆菌)制剂对小鼠肠道免疫相关分子的影响。方法将18只BALB/c小鼠随机分成对照组、益生菌培养上清组和菌液组。每只小鼠每天灌胃1次,连续处理1周。实时定量PCR检测脾脏和肠系膜淋巴结组织白细胞介素1α(IL-1α)、IL-2、IL-4、IL-6、IL-12α、IL-17A、IL-17F、IL-10、IL-18和IL-21、α干扰素(IFN-α)、IFN-β、IFN-γ、肿瘤坏死因子α(TNF-α)、转化生长因子β(TGF-β)的m RNA水平,ELISA检测血清IL-17A、IL-21水平。结果与对照组相比,喂食益生菌培养上清组小鼠脾脏组织各种细胞因子的m RNA水平无明显改变,但喂食四联活菌可增加IL-12α、IL-17A、IL-17F和IL-21的m RNA水平;喂食益生菌培养上清和喂食四联活菌均可增加肠系膜组织IL-12α、IL-17F和IL-21的m RNA水平;外周血中IL-17F的水平升高。结论益生菌处理可增加机体白细胞介素水平。
Objective To investigate the effects of four viable strains (Bulgarian bacterium, Bifidobacterium infantis, Bacillus subtilis and Lactobacillus acidophilus) on intestinal immune-related molecules in mice. Methods Eighteen BALB / c mice were randomly divided into control group, probiotic culture supernatant group and bacterial liquid group. Each mouse was given gavage once daily for 1 week. The levels of IL-1α, IL-2, IL-4, IL-6, IL-12α, IL-17A, IL-17F, IL-10 and IL-18 in the spleen and mesenteric lymph nodes were detected by real- And m RNA levels of IL-21, IFN-α, IFN-β, IFN-γ, TNF-α and TGF- -17A, IL-21 levels. Results Compared with the control group, there was no significant change in m RNA levels of various cytokines in the spleen tissue of the probiotic culture supernatants group, but the live quadriceps could increase the levels of IL-12α, IL-17A, IL-17F The level of m RNA of IL-21, IL-17 and IL-21 in mesentery could be increased by feeding probiotic culture supernatant and fed with viable bacteria. The level of IL-17F in peripheral blood increased . Conclusion Probiotic treatment can increase the body’s interleukin levels.