论文部分内容阅读
2,4-二硝基苯肼(2,4-dinitrophenylhydrazine简称2,4-DNP)是检定酮醛类化合物的常用分析试剂,但市售的试剂中常含有小分子酮醛类的2,4-二硝基苯腙的杂质。因此在使用之前必须纯化。通常采用稀盐酸或乙醇重结晶纯化,本实验室试用结果不理想,我们采用乙酸乙酯重结晶获得了满意的结果。我们以进口Chinoin试剂级2,4-DNP为纯化对象。 1.稀盐酸结晶法:2克试剂用100毫升6N盐酸回流一小时,滤去少量不溶物,冷却,
2,4-dinitrophenylhydrazine (2,4-DNP) is a commonly used assay reagent for the determination of ketoaldehydes. Commercially available reagents, however, often contain 2,4- Dinitrophenyl hydrazone impurities. Therefore must be purified before use. Usually dilute hydrochloric acid or ethanol recrystallization purification, the laboratory test results are not satisfactory, we recrystallized with ethyl acetate to obtain satisfactory results. We imported Chinoin reagent grade 2,4-DNP as the purification target. 1. Dilute hydrochloric acid crystallization method: 2 g of reagent with 100 ml of 6N hydrochloric acid reflux for one hour, filtered to remove a small amount of insolubles, cooling,