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应用形态计量学和免疫组织化学方法观察 41例少精子症和无精子症患者的睾丸活检曲细精管界膜的病理学变化。曲细精管生精上皮的病理变化主要有三种类型 :生精功能低下、生精阻滞和唯支持细胞综合征。三种病变的曲细精管均表现有不同程度的界膜增厚。形态计量学结果显示三种病变的曲细精管界膜厚度、管内径与正常对照相比均有显著性差异 ,管内径与界膜厚度呈负相关。免疫组化层粘连蛋白及Ⅳ型胶原染色显示界膜的内外层呈阳性反应 ,而中间增厚带为阴性 ,Ⅰ型、Ⅲ型胶原免疫组化染色界膜增厚的中间带为阳性。提示 :病变界膜的成分为胶原纤维 ,但与正常成分———Ⅳ型胶原不同 ,表现为Ⅰ型及Ⅲ型胶原增生
The histopathological changes of testicular biopsies in 41 patients with oligospermia and azoospermia were observed by morphometry and immunohistochemistry. Tracheal seminiferous epithelium of the pathological changes are mainly three types: dysfunctional spermatogenesis, spermatogenic arrest and only support cell syndrome. Three kinds of lesions of seminiferous tubules showed varying degrees of membrane thickening. Morphometry results showed that the three kinds of lesions of the seminiferous tubule thickness, tube diameter compared with the normal control were significantly different, the tube diameter and the boundary membrane thickness was negatively correlated. Immunohistochemistry of laminin and type Ⅳ collagen staining showed that the inner and outer layers of the membrane showed a positive reaction, while the middle thickening zone was negative. The type Ⅰ and type Ⅲ collagen immunohistochemical staining showed that the medial zone was thicker. Tip: Lesions of the membrane components of collagen fibers, but with the normal component - type Ⅳ collagen, the performance of type Ⅰ and type Ⅲ collagen hyperplasia