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目的快速提取及测定胃肠安丸中厚朴酚及和厚朴酚。方法用ASE-300型快速溶剂萃取系统进行快速萃取胃肠安丸中厚朴酚及和厚朴酚,利用反相高效液相色谱法(HPLC)测定厚朴酚及和厚朴酚的含量,Diamonsil(TM)C18柱(250 mm×4.6 mm,5μm),流动相:甲醇-水-冰醋酸(79∶21∶0.25),流速:1.0 ml/min,检测波长:294 nm,柱温∶23℃。结果HPLC法测得在范围内呈良好线性关系,和厚朴酚的平均回收率为99.91%,RSD为1.70%;厚朴酚的平均回收率为99.50%,RSD为0.83%。结论本方法简便、准确、专属性强,能有效地控制胃肠安丸的质量。
Objective To rapidly extract and determine the gavage pharmacy and honokiol. Methods The ASE-300 rapid solvent extraction system was used to rapidly extract the gavage psoriasis and honokiol and the content of magnolol and honokiol was determined by reversed-phase high performance liquid chromatography (HPLC). Diamonsil (TM) C18 column (250 mm × 4.6 mm, 5 μm), mobile phase: methanol-water-glacial acetic acid (79:21:0.25), flow rate: 1.0 ml/min, detection wavelength: 294 nm, column temperature: 23°C . Results The HPLC method showed a good linear relationship in the range. The average recovery of honokiol was 99.91% and the RSD was 1.70%. The average recovery of honokiol was 99.50%, and the RSD was 0.83%. Conclusion This method is simple, accurate, and highly specific. It can effectively control the quality of Weiwei Anwan.