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OBJECTIVE Experimental autoimmune encephalomyelitis(EAE),the classical animal model for multiple sclerosis(MS)is triggered by an impaired balance of T helper(Th)cells and regulatory T(Tregs)cells.Matrine(MAT),a quinolizidine alkaloid derived from the herb Radix Sophorae Flave,has been shown to ameliorate the clinical signs,inflammatory infiltration,demyelination in acute EAE rats.However,whether MAT protect from EAE by adjusting Th and Treg cells response in specific-cellular and molecular level is unknown.METHODS Herein,MAT was tested for its effects on Th1,Th2,Th17 and Treg cells in the spinal cord of EAE mice and splenocyte-extracted from EAE mice with MOG35-55-restimulated,respectively.RESULTS Our findings revealed that MAT significantly inhibit the proliferation of splenocyte,and remarkably down-regulate the differentiation of Th1/Th17 cells with decreased expressions of CD4+IFN-γ+cells and CD4+IL-17+cells in vivo and IL-17,IFN-γ,ROR-γt,T-bet in vitro,meanwhile it dramatically up-regulate the Th2/Treg cells response associated with increased levels of CD4+TGF-β+1cells and CD4+IL-10+cells in vivo and IL-4,IL-10,TGF-β1,Foxp3 and GATA3in vitro.CONCLUSION Considering the effective therapeutic effects of MAT on EAE,it′s worth to find its new values on other autoimmune diseases.
OBJECTIVE Experimental Autoimmune encephalomyelitis (EAE), the classical animal model for multiple sclerosis (MS) is triggered by an impaired balance of T helper (Th) cells and regulatory T (Tregs) cells. Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae Flave, has been shown to ameliorate the clinical signs, inflammatory infiltration, demyelination in acute EAE rats. Despite whether MAT protect from EAE by adjusting Th and Treg cells response in specific-cellular and molecular level is unknown. METHHODS Herein, MAT was tested for its effects on Th1, Th2, Th17 and Treg cells in the spinal cord of EAE mice and splenocyte-extracted from EAE mice with MOG35-55-restimulated, respectively. RESULTS Our findings revealed that MAT strongly inhibit the proliferation of splenocyte , and remarkably down-regulate the differentiation of Th1 / Th17 cells with decreased expressions of CD4 + IFN- γ + cells and CD4 + IL-17 + cells in vivo and IL-17, IFN- γ, ROR- γt, T-bet in vitro, meanwhile it dramatically up-regulate the Th2 / Treg cells response associated with increased levels of CD4 + TGF-β + 1 cells and CD4 + IL-10 + cells in vivo and IL-4, IL- 10, TGF- β1, Foxp3 and GATA3 in vitro. Considering the effective therapeutic effects of MAT on EAE, it’s worth to find its new values on other autoimmune diseases.