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目的探讨载脂蛋白B(ApolipoproteinB,apoB)基因XbaⅠ多态性与维吾尔族自然长寿之间的关系。方法选择新疆和田地区191名年龄90岁以上的健康维吾尔族个体为研究对象,另选53名年龄65~70岁、民族、性别、地域相匹配的正常个体为对照。采用PCR-序列特异引物(Sequencespecificprimer,PCR-SSP)、PCR-限制长度片段多态性(Restrictionfragmentlengthpolymorphism,PCR-RFLP)和PCR-直接测序(PCR-sequencing)等技术对apoB基因第26外显子XbaⅠ限制性片段长度多态性(Restrictionfragmentlengthpolymorphisms,XbaⅠ-RFLP)进行分型。结果经Logis-tic回归分析显示,长寿组apoB基因X+X+基因型频率显著高于对照组(P<0·05)。结论基因型X+X+与自然长寿显著正相关,可能为长寿的保护因素。
Objective To investigate the relationship between polymorphism of Xba Ⅰ gene of Apolipoprotein B (apoB) gene and natural longevity of Uygur nationality. Methods A total of 191 healthy Uighur individuals over the age of 90 in Hetian, Xinjiang were selected as study subjects. Another 53 normal individuals aged 65- 70 years and ethnic, gender and geographical regions were selected as controls. PCR-SSP, PCR-RFLP and PCR-sequencing were used to detect the expression of XbaⅠ in exon 26 of apoB gene Restriction fragment length polymorphisms (Xba I-RFLP) for typing. Results Logis-tic regression analysis showed that the frequency of X + X + genotype of apoB gene in longevity group was significantly higher than that in control group (P <0.05). Conclusion Genotype X + X + is significantly and positively correlated with natural longevity, which may be the protective factor of longevity.