论文部分内容阅读
本文报道血清胆红素测定的Michaelsson改良法。此法具有特异性高,溶血影响少,显色时无混浊,精确,且便于自动化测定等优点。一、原理:血清总胆红素,在二羟丙茶碱—安息香酸钠—醋酸钠催化下,与重氮试剂进行重氮反应,而血清直接胆红素在以水稀释的血清中,加重氮试剂进行重氮反应,分别生成偶氮胆红素,用左旋半胱氨酸终止反应后,加费林氏Ⅱ液,使反应液呈碱性,显色的碱性偶氮胆红素呈蓝色,于600nm波长处测其光密度,便可由标准曲线求出血清胆红素量。二、试剂:(1)二羟丙茶碱试剂:取二羟丙茶碱C_(10)H_(14)N_4O_44g,安息香酸钠C_6H_5COONa(AR)8g,加蒸馏水(预温50～60℃)约60ml,使溶解,室温冷却,加醋酸钠CH_3COONa·3H_2O12.5g,溶解,加蒸馏水至100ml。置室温可用二个月。(2)重氮试剂(Ⅰ.Ⅱ液常规配):临用前,取Ⅰ液4ml,
This article reports the determination of serum bilirubin Michaelsson improved method. This method has a high specificity, less hemolysis, no color when turbid, accurate, and easy to automate the determination of the advantages. First, the principle: serum total bilirubin, diprophylline - sodium benzoate - sodium acetate catalysis, and diazo reagent diazo reaction, and serum direct bilirubin in water diluted serum, increased Nitrogen reagent diazo reaction, respectively, generated azo bilirubin, with cysteine termination reaction, add Fellings Ⅱ liquid, the reaction was alkaline, the color of the basic azo-bilirubin was Blue, measured at 600nm wavelength optical density, can be obtained from the standard curve serum bilirubin. Reagents: (1) Dihydroxypropylamine reagent: Take diphenylamine C_ (10) H_ (14) N_4O_44g, sodium benzoate C_6H_5COONa (AR) 8g, add distilled water (preheat 50 ~ 60 ℃) about 60ml, dissolved, cooled at room temperature, add sodium acetate CH_3COONa · 3H_2O12.5g, dissolved, add distilled water to 100ml. Room temperature available for two months. (2) diazo reagent (Ⅰ.Ⅱ liquid conventional with): before use, take Ⅰ liquid 4ml,