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为筛选出抗迟缓爱德华菌的牙鲆家系,实验以已经选育出的牙鲆抗病和快速生长家系以及从韩国引进的选育群体为亲本建立了牙鲆家系56个,并对其中32个家系进行了迟缓爱德华菌人工感染实验。确定了迟缓爱德华菌对牙鲆家系的半致死浓度LD50为3.69×105CFU/mL后,从每个家系中随机抽取75尾,按照0.2 mL/10 g体质量腹腔注射半致死浓度的菌液,并设置1次重复。人工感染时水温控制在(19±1)℃。从32个家系中共选取4 800尾5月龄牙鲆幼鱼进行感染,16 d实验结束后统计各家系存活率为8.2%~66.1%,平均存活率为31.2%。不同家系对迟缓爱德华菌的抗感染能力表现出显著差异(P<0.05)。筛选出6个存活率高于45%的抗病家系,发现2007年筛选出的抗鳗弧菌病家系F0768的后代家系在迟缓爱德华菌感染后的存活率普遍很高,表现出抗迟缓爱德华菌病的能力。研究为选育抗鳗弧菌病和抗迟缓爱德华菌病的牙鲆优良品系奠定了基础,对牙鲆抗细菌病的分子机理研究及抗病新品种选育具有重要意义和应用价值。
In order to select the pedigree of flounder Paralichthys olivaceus, the experiment was to establish 56 pedigrees of Paralichthys olivaceus (Paralichthys olivaceus) families with resistant and rapidly growing Paralichthys olivaceus families and breeding population introduced from South Korea, and 32 Pedigree conducted a slow infection of Edwardella infection experiment. After determining the LD50 of S. edwardsiana to Paralichthys olivaceus family was 3.69 × 105CFU / mL, 75 individuals were randomly selected from each pedigree, and the LC50 was injected intraperitoneally at 0.2 mL / 10 g body weight. Set 1 repeat. Artificial infection water temperature control (19 ± 1) ℃. A total of 4 800 larvae of 5-month-old flounder were selected from 32 pedigrees for infection. After 16 days of experiment, the survival rate of each line was 8.2% -66.1%, and the average survival rate was 31.2%. Different families showed significant differences in the anti-infective capacity of S. edwardsiana (P <0.05). Six resistant families with more than 45% survival rate were screened out. The progeny lines of F0768, a strain of Vibrio anguillarum screening in 2007, were found to be highly resistant to Edwardsiella spp. Sickness. The study laid the foundation for the selection of excellent strains of flounder with resistance to Vibrio anguillarum and Edwardsiella spp., Which has important significance and application value for the study of the molecular mechanism of anti-bacterial disease and breeding of disease-resistant new varieties.