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目的:探讨骨髓间充质干细胞移植对阿霉素诱导的心力衰竭大鼠心功能及心肌细胞基质金属蛋白酶(MMP)-9及其抑制因子(TIMP)-1表达的影响。方法:Wister大鼠分为正常对照组、心力衰竭对照组和细胞移植组,后2组腹腔注射阿霉素建立扩张型心肌病模型。细胞移植组尾静脉注射骨髓间充质干细胞。移植后4周,超声心动图测量左室射血分数,免疫组化检查MMP-9、TIMP-1表达情况。结果:与心力衰竭对照组比较,细胞移植组LVEF和MMP-9明显增高,TIMP-1明显下降(P<0.05),接近正常对照组(P>0.05)。结论:经静脉移植骨髓间充质干细胞能改善心力衰竭大鼠心功能,调节MMP-9/TIMP-1表达,影响心室重塑。
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell transplantation on cardiac function and expression of matrix metalloproteinase (MMP) -9 and TIMP-1 in adriamycin-induced heart failure rats. Methods: Wistar rats were divided into normal control group, heart failure control group and cell transplantation group. The latter two groups were injected with doxorubicin to establish dilated cardiomyopathy model. Bone marrow-derived mesenchymal stem cells were injected into the tail vein of the cell transplantation group. Four weeks after transplantation, left ventricular ejection fraction was measured by echocardiography and the expression of MMP-9 and TIMP-1 by immunohistochemistry. Results: Compared with the control group, LVEF and MMP-9 in the cell transplantation group were significantly increased and TIMP-1 was significantly decreased (P <0.05), close to the normal control group (P> 0.05). Conclusion: Bone marrow mesenchymal stem cells can improve cardiac function, regulate MMP-9 / TIMP-1 expression and affect ventricular remodeling in rats with heart failure.