论文部分内容阅读
目的:研究爱普列特对体外培养前列腺上皮细胞表皮生长因子受体和胰岛素样生长因子-Ⅰ受体表达的影响,探讨其抗前列腺增生的分子机制。方法:MTT法检测爱普列特对外源性表皮生长因子(epider-mal growth factof,EGF),胰岛素样生长因Ⅰ(insulin-like growth factor Ⅰ,IGF-Ⅰ)诱导的大鼠前列腺上皮细胞增殖的作用,逆转录PCR及流式细胞术定量检测体外培养前列腺上皮细胞EGFR和IGF-ⅠR mRNA及蛋白表达。结果:爱普列特180nmol/L和360nmol/L可以明显抑制IGF-Ⅰ5,25μg/L诱导的细胞增殖;爱普列特360 nmol/L可以抑制EGF25μg/L诱导的细胞增殖;爱普列特360nmol/L可以明显下调EGFR、IGF-ⅠR mRNA的表达,而爱普列特180nmol/L则没有明显作用;爱普列特180nmol/L和360nmol/L均可以明显下调EGFR和IGF-ⅠR蛋白的表达。结论:爱普列特抗前列腺增生的分子机制与纠正增生时异常表达的生长因子有关。
OBJECTIVE: To study the effect of Epsilon on the expression of epidermal growth factor receptor and insulin-like growth factor-1 receptor in cultured prostatic epithelial cells in vitro and to explore its molecular mechanism of anti-benign prostatic hyperplasia. Methods MTT assay was used to detect the proliferation of rat prostatic epithelial cells induced by epidermal growth factor (epidermal growth factor of EGF) and insulin-like growth factor Ⅰ (IGF-Ⅰ) RT-PCR and flow cytometry were used to detect the expression of EGFR and IGF-ⅠR mRNA and protein in cultured prostate epithelial cells. Results: Epristeride 180nmol / L and 360nmol / L could significantly inhibit IGF-Ⅰ5,25μg / L induced cell proliferation; Cyproconazole 360nmol / L can inhibit EGF25μg / L induced cell proliferation; 360nmol / L could obviously downregulate the expression of EGFR and IGF-ⅠR mRNA, but there was no significant effect of 180nmol / L of epristeride. Both 180nmol / L and 360nmol / L of epristeride significantly downregulated the expression of EGFR and IGF- expression. CONCLUSIONS: The molecular mechanism of Epstein Anti-Prostatic Hyperplasia correlates with the correction of abnormally expressed growth factors during hyperplasia.