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目的 在大鼠小肠缺血再灌注（ＩＩＲ） 模型上，研究内源性一氧化氮（ＮＯ）在ＩＩＲ所致肺损伤发病过程中的作用；观察大黄对ＮＯ的影响，探讨大黄防治肠源性肺损伤的机理。方法 ＳＤ大鼠随机分为肠缺血再灌注组、假手术组、大黄治疗组和安慰剂组监测平均动脉压（ＭＡＰ）。以１２５Ｉ标记小牛血清白蛋白（ＢＳＡ） 肺摄取指数作为评价肺毛细血管通透性的指标；采用镉还原柱层析和比色法分别测定各组动物不同时间血浆、肺及小肠组织内源性ＮＯ的含量。结果 大黄可明显改善ＩＩＲ导致的低血压状态；抑制血浆、肺及小肠组织内源性ＮＯ的释放（ Ｐ＜ ０－０５ 或Ｐ＜０－０１）；降低肺毛细血管通透性（ Ｐ＜ ０－０１） 。结论 早期应用大黄有助于防止大鼠肠源性肺损伤的发生，这种作用部分是通过抑制内源性ＮＯ大量释放实现的。
Objective To investigate the role of endogenous nitric oxide (NO) in the pathogenesis of IIR-induced lung injury in rat model of intestinal ischemia-reperfusion (IIR). To observe the effect of rhubarb on NO, Mechanism of lung injury. Methods SD rats were randomly divided into intestinal ischemia reperfusion group, sham operation group, rhubarb treatment group and placebo group to monitor mean arterial pressure (MAP). 125I-labeled bovine serum albumin (BSA) pulmonary uptake index was used as an index to evaluate pulmonary capillary permeability. Cadmium reduction column chromatography and colorimetric method were used to determine the endogenous levels of plasma, lung and small intestine Sexual NO content. Results Rhubarb significantly improved the hypotensive state caused by IIR, inhibited the release of endogenous NO in plasma, lung and small intestine (P <0-05 or P <0-01), and decreased pulmonary capillary permeability (P <0 -01). Conclusion The early application of rhubarb helps to prevent the development of intestinal-induced lung injury in rats. This effect is partly achieved by inhibiting the massive release of endogenous NO.