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目的:探讨血管紧张素II(angiotensinⅡ,AngII)、去甲肾上腺素(norepinephrine,NE)对心肌细胞增殖、胚胎基因心钠素及转化生长因子β1(transforminggrowthfactorβ1,TGF-β1)表达的影响及其生物学意义。方法:体外培养的乳鼠心肌细胞,分别以AngII(1×10-6mol/L)、NE(1×10-6mol/L)刺激后,采用流式细胞计数仪检测心肌细胞DNA合成、细胞周期分布;免疫细胞化学检测心肌细胞内TGF-β1表达水平,放射免疫学检测细胞上清心钠素浓度。结果:与对照组相比,AngII,NE刺激24h后,心肌细胞心钠素表达增加,G0/G1,G2/M期DNA合成增强,同时伴有TGF-β1蛋白表达上调(P均<0.05),但二者不改变心肌细胞的细胞周期分布,细胞仍主要处于G0/G1期。结论:AngII,NE能诱导心肌细胞异常增生,但不能刺激细胞增殖。这可能与AngII,NE诱导心肌细胞TGF-β1表达增加有关。
Objective: To investigate the effects of angiotensin Ⅱ (AngII) and norepinephrine (NE) on the proliferation of cardiomyocytes, the expression of atrial natriuretic peptide and transforming growth factor β1 (TGF-β1) Significance of learning. Methods: The neonatal rat cardiomyocytes cultured in vitro were stimulated with AngII (1 × 10-6 mol / L) and NE (1 × 10-6 mol / L) respectively, and the DNA synthesis and cell cycle of cardiomyocytes were detected by flow cytometry Distribution of TGF-β1 expression in cardiomyocytes was detected by immunocytochemistry, and atrial natriuretic peptide concentration was detected by radioimmunoassay. Results: Compared with the control group, the expression of atrial natriuretic peptide increased after 24 hours of AngII and NE stimulation, and the DNA synthesis increased at G0 / G1 and G2 / M phase accompanied with the up-regulation of TGF-β1 protein expression (all P <0.05) , But the two did not change the cell cycle distribution of cardiomyocytes, cells still mainly in the G0 / G1 phase. Conclusion: AngII and NE can induce cardiomyocyte dysplasia, but can not stimulate cell proliferation. This may be related to AngII, NE-induced increased expression of TGF-β1 in cardiomyocytes.