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目的:研究薏苡仁多糖不同组分对脾虚水湿不化模型大鼠血清VIP、ADH、ALD的影响,探讨薏苡仁多糖改善机体水液代谢的作用机制。方法:采用高脂低蛋白饮食加力竭游泳法复制脾虚水湿不化大鼠模型,水负荷实验检测水负荷指数和6 h尿量,采用ELISA方法观察薏苡仁多糖不同组分对模型大鼠血清VIP、ADH、ALD的影响。结果:给药干预后,与模型组相比,各用药组大鼠水负荷指数明显降低(P<0.05),尿量明显增多(P<0.01);薏苡仁粗多糖组和薏苡仁多糖组分Ⅰ组大鼠血清VIP、ADH、ALD明显降低(P<0.05)。结论:薏苡仁多糖不同组分能改善脾虚水湿不化大鼠水液代谢功能,其中薏苡仁多糖组分Ⅰ作用最明显,其作用机理与降低血清VIP、ADH、ALD水平有关。
Objective: To study the effects of different components of Coixan on serum VIP, ADH and ALD in rats with spleen-asthenia-dampness syndrome and to explore the mechanism of Coixan polysaccharide in improving the body’s fluid-water metabolism. Methods: High fat and low protein diet plus exhaustive swimming method was used to replicate spleen deficiency water wet rat model. The water load test was used to detect the water load index and urine output for 6 hours. The effects of different components of Coixan were observed in model rats Serum VIP, ADH, ALD. Results: Compared with the model group, the water load index of rats in each treatment group was significantly lower (P <0.05) and the urine output was significantly increased (P <0.01) Serum VIP, ADH and ALD in group Ⅰ were significantly decreased (P <0.05). CONCLUSION: Different components of Coixan improve the water-fluid metabolism in rats with spleen-asthenia-dampness syndrome, and the role of Coixan polysaccharide component I is the most obvious. Its mechanism of action is related to the reduction of serum VIP, ADH and ALD levels.