论文部分内容阅读
第三届病毒快速诊断新方法专题会议于1983年12月29～30日在美国国家卫生研究院召开。内容有关于核酸杂交法的应用、同型特异性血清学试验、检测免疫反应的新技术以及单克隆抗体等。一、核酸杂交法 Wahl回顾了核酸杂交的各种方法,包括:(1)DNA转移技术;(2)未组分的斑点或狭孔(slot)杂交技术;(3)原化杂交技术。他描述了在固相支持物上,影响用部分重叠的缺口翻译探针作杂交的一些参数。建议用单股探针处理杂交前溶液中探针的重退火问题,但单股探针并不形成互补探针能形
The Third Symposium on New Methods of Rapid Virus Diagnosis was held at the National Institutes of Health in the United States on December 29-30, The content of the application of nucleic acid hybridization method, the same type of specific serological tests, detection of immune response of new technologies and monoclonal antibodies. 1. Nucleic Acid Hybridization Methods Wahl reviews various methods of nucleic acid hybridization, including: (1) DNA transfer techniques; (2) uncompressed spot or slot hybridization techniques; and (3) an in situ hybridization technique. He described some of the parameters that affect hybridization with partially overlapping gapped translation probes on solid supports. It is advisable to deal with the problem of re-annealing of probes in a pre-hybridization solution with a single-stranded probe, but single-stranded probes do not form complementary probe energies