论文部分内容阅读
干扰素(IFNs)是一族具有多种生物活性的蛋白质,在机体防御病毒感染中起重要作用。其中γ-干扰素(IFNγ)由 T 淋巴细胞及经白细胞介素Ⅰ(IL-2)作用后的自然杀伤细胞产生。作者应用夹心酶联免疫吸附技术对50例慢性肝病患者(无症状 HBsAg 携带者5例,慢迁肝12例,慢活肝17例;肝硬化16例),其中非甲非乙型肝炎18例,作 IFNγ测定,以估价IFN 系统在慢性肝病发病中的作用。20名健康成人作为对照组。所有研究对象均未用过免疫抑制剂治疗。取外周血单核细胞(PBMCs)与刀豆素 A(Con-A),或 IL-2、消炎痛在 RPMI-1640培养基中培养72小时,离心取
Interferons (IFNs) are a family of proteins that have a variety of biological activities that play an important role in the defense of the body against viral infections. Among them, γ-interferon (IFNγ) is produced by T lymphocytes and natural killer cells after the action of interleukin-1 (IL-2). Fifty patients with chronic liver disease (5 asymptomatic HBsAg carriers, 12 slow-moving liver, 17 slow-acting liver and 16 liver cirrhosis) were selected by sandwich enzyme-linked immunosorbent assay (ELISA), including 18 non-A non-B hepatitis For IFNγ measurement to assess the role of IFN system in the pathogenesis of chronic liver disease. Twenty healthy adults served as control group. None of the subjects used immunosuppressive therapy. Peripheral blood mononuclear cells (PBMCs) were incubated with Con-A or IL-2 and indomethacin in RPMI-1640 medium for 72 hours. Centrifuge