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目的探讨丙烯酰胺(ACR)中毒后脑能量代谢的改变。方法采用酶分析法测定O1a鼠和6J鼠脑组织匀浆中肌酸激酶(CK)、三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、腺苷酸(AMP)和葡萄糖含量。结果O1a鼠上述指标改变轻微。6J鼠ATP水平明显降低,达1.76μmol/g,与对照组(2.53μmol/g)比较,差异有显著性(P<0.01);ADP和AMP增加,葡萄糖降低,脑能量负荷值降低,CK活力明显受到抑制,低达1.13μmol/g,与对照组(3.16μmol/g)比较,差异有显著性(P<0.01),且持续到第5周不恢复。结论ACR中毒对Ola鼠脑能量代谢影响轻微且可逆,对6J鼠产生严重影响且持续时间长;脑组织供能代偿潜能损伤重,这种能量代谢的抑制是ACR产生神经元损伤的生化基础。
Objective To investigate the changes of brain energy metabolism after acrylamide (ACR) poisoning. Methods The levels of creatine kinase (CK), adenosine triphosphate (ATP), adenosine monophosphate (ADP), adenosine monophosphate (AMP) and glucose in brain homogenate of O1a and 6J mice were determined by enzyme assay. Results O1a mice changed slightly. (P <0.01). Compared with the control group (2.53μmol / g), the level of ATP was significantly decreased (P <0.01). The ADP and AMP increased, the glucose decreased, the brain energy load decreased, Was significantly inhibited, as low as 1.13μmol / g, compared with the control group (3.16μmol / g), the difference was significant (P <0.01), and continued until the 5th week does not recover. Conclusion ACR intoxication has a slight and reversible effect on energy metabolism of brain in Ola rats, which has a serious effect on 6J rats and lasted for a long time. The impairment of energy supply in brain tissue is severely impaired, and the inhibition of energy metabolism is the biochemical basis of ACR-induced neuronal injury .