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目的研究ＨＢＶ感染在小儿膜性肾炎的致病作用。方法３２例肾穿刺活组织检查诊为膜性肾炎的小儿作为研究对象。所有病例检测血清ＨＢＶ感染标志物，２９例应用免疫组化技术检测肾组织ＨＢＶ抗原，１２例应用Ｓｏｕｔｈｅｒｎ印迹杂交检测肾组织ＨＢＶＤＮＡ，采用３２Ｐα－ｄＣＴＰ标记的探针；２８例应用原位杂交检测肾组织ＨＢＶＤＮＡ，采用地高辛标记的探针。结果三种方法综合分析，３２例膜性肾炎中，肾组织ＨＢＶ抗原和（或）ＨＢＶＤＮＡ阳性者共３０例，达９３．７４％。结论进一步证实ＨＢＶ感染在我国小儿膜性肾炎起主要致病作用
Objective To study the pathogenic role of HBV infection in children with membranous nephritis. Methods 32 children with membranous nephritis diagnosed as renal biopsy were included in this study. Serum HBV infection markers were detected in all cases, 29 cases of renal tissue HBV antigen detected by immunohistochemistry, 12 cases of HBVDNA were detected by Southern blot hybridization with 32Pα-dCTP labeled probe; 28 cases detected by in situ hybridization Tissue HBVDNAs were labeled with digoxigenin. Results The three methods of comprehensive analysis, 32 cases of membranous nephritis, renal tissue HBV antigen and (or) HBVDNA positive in a total of 30 cases, up to 93.74%. Conclusions further confirmed that HBV infection plays a major role in the pathogenesis of pediatric membranous nephritis in our country