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以大田试验获得的大麦氮敏感基因型BI-45为材料,利用溶液培养方法,测定了苗期株高、根长、叶绿素含量、含氮量、谷氨酰胺合成酶和硝酸还原酶活性,以及与氮代谢相关的基因(GS1_1、GS1_2、GS1_3、GS2、Nar1、NRT2.1、NRT2.2、NRT2.3和NRT2.4)的表达。结果表明:相对于正常供氮,氮饥饿胁迫下,BI-45根和叶中的氮素利用率提高,含氮量降低,叶绿素含量减少,根冠比增加;叶片中的谷氨酰胺合成酶活性和硝酸还原酶的活性高于根,但是,与叶中的相比,根中的谷氨酰胺合成酶活性升高及硝酸还原酶活性降低的差异性更显著;与正常供氮相比,氮饥饿处理下,根中基因GS家族,基因Nar1和硝酸盐转运蛋白基因NRT2家族的相对表达量皆达到显著性差异,其中GS1_1、GS1_2和NRT2.2在苗期大麦氮饥饿处理下表现尤为突出,并且在6 h都有上调表达。
The plant height, root length, chlorophyll content, nitrogen content, glutamine synthetase and nitrate reductase activity in seedling stage were determined by using the solution culture method. Expression of genes related to nitrogen metabolism (GS1_1, GS1_2, GS1_3, GS2, Nar1, NRT2.1, NRT2.2, NRT2.3 and NRT2.4). The results showed that compared with the normal nitrogen supply, under nitrogen starvation stress, the nitrogen utilization rate in roots and leaves of BI-45 increased, the nitrogen content decreased, the content of chlorophyll decreased, and the ratio of root to shoot increased. The content of glutamine synthetase Activity and nitrate reductase activity were higher than those of roots, but the difference of glutamine synthetase activity and nitrate reductase activity was more significant in roots than in leaves. Compared with normal nitrogen supply, Under nitrogen starvation, the relative expression levels of GS gene, Nar1 gene and NRT2 gene of nitrate transporter gene all reached significant differences, among which GS1_1, GS1_2 and NRT2.2 were most prominent under nitrogen starvation in seedling stage , And up-regulated expression at 6 h.