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目的 :观察健脑安 (CGE)对脑缺血 再灌注大鼠高表达的IL 1β ,c fos蛋白的抑制作用。 方法 :采用左侧大脑中动脉插入丝线结扎方法制造大鼠血管性痴呆模型 ,分成健脑安大 (19.34× 10 3 g·L-1生药 )、中 (9.6 7× 10 3 g·L-1生药 )、小 (4.83× 10 3 g·L-1生药 )剂量组、IL 1ra对照组、假手术组和模型组。中药用 0 .5 %羧甲基纤维素 (CMC)配成水溶液 ,每 10 0g大鼠灌胃给中药溶液 0 .7mL。IL 1ra对照组大鼠在缺血前 30min和缺血后 10min左侧脑室内分别注射rhIL 1ra 10 μg,假手术组注射同等剂量生理盐水 10 μL。假手术组、模型组分别于造模 7d前开始给予中药灌胃 ,分别按照缺血再灌后不同时间点 (0 .5 ,1,2 ,3,4 ,6 ,8,12 ,2 4 ,4 8,72 ,96 ,14 4 ,16 8h)取材。应用免疫组化方法标记实验各组大鼠应用健脑安和IL 1ra后脑组织中海马区IL 1β蛋白和c fos阳性神经元的数量 ,进行组间均数比较。 结果 :与模型组相比 ,健脑安 3个剂量组在不同再灌时间 (2 ,3,4 ,12 ,72h)均具有显著性的抑制IL 1β ,c fos蛋白表达的作用 ,但小、中剂量组的抑制水平不及IL 1ra(P <0 .0 5和P <0 .0 1)。健脑安抑制海马IL 1β蛋白表达 ,2h起效 ,大剂量 (5 31± 15 1.1)和中剂量 (5 89.3± 78.6 )都强于模型组 (6 87.6
Objective: To observe the inhibitory effect of Jiannaoan (CGE) on high expression of IL 1β and c fos in rats with cerebral ischemia-reperfusion. METHODS: A rat model of vascular dementia was created by inserting the left middle cerebral artery into the silk ligation method and divided into Jiannao Andalou (19.34×10 3 g·L-1 crude drug) and Medium (9.6 7×10 3 g·L-1). The crude drug), the small (4.83×10 3 g·L-1 crude drug) dose group, the IL 1ra control group, the sham operation group, and the model group. Chinese herbal medicines were prepared with 0.5% carboxymethyl cellulose (CMC) as an aqueous solution. Each 100 g rat was given orally 0.7 mL of Chinese medicine solution. In the IL 1ra control group rats, rhIL 1ra 10 μg was injected into the left cerebral ventricle 30 min before ischemia and 10 min after ischemia, and 10 μL of normal saline was injected into the sham group. The rats in the sham operation group and the model group were given intragastric administration of gavage medicine 7 days before model establishment, respectively according to different time points after ischemia and reperfusion (0.5, 1, 2, 3, 4, 6, 8, 12, 24, respectively). 4 8,72,96,14 4,16 8h) The number of IL-1β protein and c fos-positive neurons in the hippocampus of the brains of the rats treated with Jianaoan and IL 1ra were marked by immunohistochemistry. The mean of the groups was compared. Results: Compared with the model group, Jiannaoan 3 dose groups had significant inhibitory effects on the expression of IL 1β and c fos proteins at different reperfusion times (2, 3, 4, 12 and 72 hours), but The inhibitory level of the middle dose group was lower than that of IL 1ra (P <0.05 and P <0.01). Jianna’an inhibited the expression of IL-1β protein in the hippocampus and was activated at 2h. Both high-dose (5 31±15 1.1) and middle-dose (5 89.3±78.6) were stronger than the model group (6 87.6).