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目的研究不同浓度氟对大鼠丙二醛(MDA)含量、肝功及光镜结构的影响。方法在饮水中加入不同剂量的氟化钠喂饲大鼠1月后,测定血清氟含量、尿氟含量、肝组织中MDA含量、血清门冬氨酸氨基转移酶(AST)及丙氨酸氨基转移酶(ALT)的活力。结果与对照组相比,各剂量组大鼠血氟浓度相应增加,存在剂量-效应关系(R=0.884,P﹤0.01),但各剂量组间血氟浓度差异无统计学意义;各剂量组大鼠尿氟浓度相应增加,与对照组比较差异有统计学意义(P﹤0.01),存在剂量-效应关系(R=0.921,P﹤0.01);各剂量组大鼠MDA含量升高,与对照组比较差异有统计学意义(P﹤0.01),存在剂量-效应关系(R=0.797,P﹤0.01);各剂量组大鼠AST含量逐渐增加,与对照组比较差异有统计学意义(P﹤0.01),存在剂量-效应关系(R=0.889,P﹤0.01);各剂量组大鼠ALT含量逐渐增加,与对照组比较差异有统计学意义(P﹤0.01),存在剂量-效应关系(R=0.729,P﹤0.01);肝细胞光镜结构改变。结论过量氟可导致大鼠肝细胞发生脂质过氧化作用。
Objective To study the effects of different concentrations of fluoride on malondialdehyde (MDA) content, liver function and light microscopic structure in rats. Methods One week after adding different doses of sodium fluoride into drinking water, the levels of serum fluoride, urinary fluoride, MDA in liver tissue, serum aspartate aminotransferase (AST) and alanine amino Transferase (ALT) activity. Results Compared with the control group, there was a dose-response relationship between the two groups (R = 0.884, P <0.01), but there was no significant difference in the blood fluoride levels between the various dose groups Compared with the control group, there was a significant difference (P <0.01) in dose-effect relationship (R = 0.921, P <0.01); the content of MDA in each dose group increased, (P <0.01). There was dose-effect relationship (R = 0.797, P <0.01). The content of AST in each dose group increased gradually, and there was significant difference compared with the control group (P < 0.01). There was a dose-effect relationship (R = 0.889, P <0.01). The ALT levels of rats in each dose group increased gradually compared with the control group (P <0.01) = 0.729, P <0.01). The light microscope of liver cells changed. Conclusion Excessive fluoride can cause lipid peroxidation in rat hepatocytes.