论文部分内容阅读
目的和方法：抑瘤素－Ｍ是一种具有多种生物活性的细胞因子，采用ＰＣＲ的方法在ＯＳＭ全长的ｃＤＮＡ基础上截去编码Ｃ端的３１个氨基酸的核苷酸序列，将其分别克隆至ｐＢＶ２２０和ＴｈｉｏＦｕｓｉｏｎＴＭ表达系统中进行表达，并对其包涵体进行初步的变性、复性和活性测定。结果和结论：证实了截去Ｃ端３１个氨基酸之后，ＯＳＭ仍保持其抑制活性。构建重组质粒ｐＢＶ２２０－ＯＳＭ、ｐＴＦ－ＯＳＭ，并得到了占全菌蛋白１５％左右的融合表达
Objective and Methods: Oncostatin-M is a cytokine with a variety of biological activity. Based on the full-length cDNA of OSM, a 31 amino acid nucleotide sequence encoding the C-terminal was truncated by PCR Cloned into pBV220 and ThioFusionTM expression system for expression, and initial inclusion, denaturation, refolding and activity determination. RESULTS AND CONCLUSION: OSM still maintained its inhibitory activity after truncating the C-terminal 31 amino acids. The recombinant plasmid pBV220-OSM, pTF-OSM was constructed and a fusion expression of about 15% of the total bacterial protein was obtained