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应用风疹病毒－聚合酶性反应（RV－PCR）技术对503例孕10－20周无风疹症状的妊娠妇女及460例非孕妇女血液标本进行风疹病毒RV－RNA测定，对孕妇RV－RNA阳性者进而检测其羊水中的RV－RNA，对胎儿风疹病毒先天性感染作出产前诊断。结果：503例孕妇血液标本中RV－RNA阳性者26例，检出率为5．17％，460例对照组非孕妇女血液标本中RV－RNA阳性者5例，检出率为1．09％，两者差异具有极显著意义（P＜0．001）。揭示孕妇是风疹病毒的易感人群。对26例外周血RV－RNA阳性的孕妇，其中20例羊术检出RV－RNA占76．92％。另外6例羊水中未检出RV－RNA。对这20例孕妇引产后追踪检测的结果与产前诊断符合。该方法能预防RV母婴垂传播和由此引起的先天畸形。
RV-PCR was used to detect RV-RNA of 503 rubella pregnant women and 460 non-pregnant women with rubella-free symptoms for 10-20 weeks. RV-RNA was positive for pregnant women Then further detection of their RV-RNA in amniotic fluid, prenatal diagnosis of fetal rubella congenital infection. Results: The positive rate of RV-RNA in blood samples of 503 pregnant women was 26 cases (5.17%). The positive rate of RV-RNA in blood samples from 460 pregnant women was 5 %, There is a significant difference between the two (P <0.001). Reveal that pregnant women are susceptible to rubella virus. Among 26 pregnant women with positive RV-RNA in peripheral blood, RV-RNA was detected in 20 of them (76.92%). RV-RNA was not detected in the other 6 cases of amniotic fluid. The results of follow-up testing of these 20 pregnant women after induction of labor were consistent with prenatal diagnosis. This method can prevent RV maternal and child transmission and the resulting congenital malformations.