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目的:探讨花姜酮(zerumbone)对人胰腺癌细胞株BxPC-3和Panc-1迁移和侵袭的影响及其相关基因的表达。方法:以BxPC-3和Panc-1为研究对象,通过四甲基偶氮唑盐(MTT)实验选择毒性较小的花姜酮浓度,用细胞划痕实验、Transwell小室迁移和侵袭实验检测药物对细胞迁移和侵袭能力的影响,蛋白质印迹(Western blot)检测各细胞组CXC族趋化因子受体4(CXCR4)、促分裂原活化蛋白酶激酶1/2(MEK1/2)、磷酸化MEK 1/2(p-MEK1/2)、细胞外调节激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)蛋白表达变化。结果:花姜酮对BxPC-3和Panc-1细胞的生长均有抑制作用,且随着药物浓度的增高和作用时间的延长而增强(P<0.05)。低浓度药物作用后BxPC-3和Panc-1的划痕迁移率都低于对照组(P<0.05)。与对照组相比,Transwell迁移和侵袭实验中BxPC-3和Panc-1用药组的平均穿膜细胞数均减少(P<0.05)。蛋白质印迹检测结果表明:花姜酮抑制CXCR4、p-MEK1/2、p-ERK1/2蛋白的表达。结论:花姜酮具有抑制胰腺癌细胞株BxPC-3和Panc-1迁移和侵袭的作用,可能与下调CXCR4、p-MEK1/2、p-ERK1/2表达水平有关,为肿瘤的靶向治疗提供新的依据。
Objective: To investigate the effects of zerumbone on the migration and invasion of human pancreatic cancer cell lines BxPC-3 and Panc-1 and the related gene expression. Methods: BxPC-3 and Panc-1 were selected as the target. Tetracycline (MTT) was used to select the less toxic concentration of zingiberone. The cell scratch assay, Transwell chamber migration and invasion assay On CXC chemokine receptor 4 (CXCR4), mitogen-activated proteinase kinase 1/2 (MEK1 / 2) and phosphorylated MEK 1 in each cell group were detected by Western blot. / 2 (p-MEK1 / 2), extracellular regulated kinase 1/2 (ERK1 / 2) and phosphorylated ERK1 / 2 (p-ERK1 / 2) Results: Zingiberone inhibited the growth of BxPC-3 and Panc-1 cells, and increased with the increase of drug concentration and prolongation of action time (P <0.05). Scratch mobility of BxPC-3 and Panc-1 was lower than that of the control group (P <0.05). Compared with the control group, the average number of transmembrane cells in BxPC-3 and Panc-1 transdermal migration and invasion assays were decreased (P <0.05). Western blotting results showed that zingerone inhibited the expression of CXCR4, p-MEK1 / 2 and p-ERK1 / 2. Conclusion: Zingiberone can inhibit the migration and invasion of pancreatic cancer cell lines BxPC-3 and Panc-1, which may be related to the downregulation of CXCR4, p-MEK1 / 2 and p-ERK1 / 2 expression, Provide a new basis.