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将一１２ａａ的ＣＴＢ表位连接于丙型肝炎病毒（Ｈｅｐａｔｉｔｉｓ Ｃ ｖｉｒｕｓ，ＨＣＶ）复合多表位抗原基因ＰＣＸ的５’端，再克隆于真核表达载体ｐｃＤＮＡ３中，获得重组质粒ｐｃＤＮＡ３／ＣＴＢ－ＰＣＸ，利用表达ＩＬ－１２的ｐＷＲＧ／ｍＩＬ－１２作为佐剂，肌注免疫小鼠后６Ｗ，用重组蛋白ＣＺ－ＰＣＸ加强免疫１次，第６Ｗ时叶检测到较高水平的抗ＧＺ－ＰＣＸＩｇＧ，最高滴度达１：１０３，与对照ｐｃＤＮＡ３／ＰＣＸ相比无显著升高，提示ＣＴＢ表位并无明显的增强ＰＣＸ 基因特异性体液免疫应答的作用。重组蛋白ＧＺ－ＰＣＸ可有效提高抗体水平，促进ＣＤ８＋Ｔ细胞增殖。免疫小鼠叶诱发钉对ＧＺ－ＰＣＸ融合蛋白的迟发性超敏反应（ＤＴＨ），免疫后小鼠体重正常，肝脾未见明显肿大，具有良好安全性。
The CTB epitope of 12aa was ligated to the 5 ’end of the PCX gene of hepatitis C virus (HCV) and then cloned into the eukaryotic expression vector pcDNA3 to obtain the recombinant plasmid pcDNA3 / CTB-PCX The mice were immunized intraperitoneally with the recombinant protein CZ-PCX for 6 weeks after immunization with pWRG / mIL-12 expressing IL-12 as an adjuvant. The mice were immunized once with the recombinant protein CZ-PCX and the higher level of anti-GZ-PCXIgG was detected at the 6th hour. The highest titer of 1: 103 was not significantly higher than that of control pcDNA3 / PCX, suggesting that CTB epitopes did not significantly enhance the PCX-specific humoral immune response. Recombinant protein GZ-PCX can effectively increase antibody levels and promote CD8 + T cell proliferation. Delayed hypersensitivity reaction (DTH) of GZ-PCX fusion protein was induced by leaf-induced spikes in immunized mice. The body weight of mice immunized was normal, and no significant enlargement was found in the liver and spleen. It possessed good safety.