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本文报道利用紫外交联法来测定一种受雄激素调控的未知的ＤＮＡ结合蛋白Ｃ３Ｐ４的亚基组成及分子量。紫外交联分析结果表明Ｃ３Ｐ４可能具有分子量约为１１０ｋＤ和２７ｋＤ的两种亚基。对Ｃ３Ｐ４进行Ｆｅｒｇｕｓｏｎ作图分析，揭示了Ｃ３Ｐ４天然分子量约为１２７ｋＤ。这表明Ｃ３Ｐ４很可能是由分子量约为１１０ｋＤ和２７ｋＤ的两个亚基组成的异二聚体，其中１１０ｋＤ在结合ＤＮＡ时起主要作用。
This paper reports the determination of the subunit composition and molecular weight of an androgen-regulated unknown DNA-binding protein C3P4 by UV-crosslinking. UV cross-linking analysis showed that C3P4 may have two subunits with molecular weights of about 110kD and 27kD. Ferguson mapping analysis of C3P4 revealed that C3P4 had a native molecular weight of about 127 kD. This suggests that C3P4 is likely to be a heterodimer composed of two subunits with molecular weights of about 110 kD and 27 kD, of which 110 kD plays a major role in DNA binding.