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目的 探讨新疆南部地区维吾尔族妇女宫颈癌组织中人乳头状瘤病毒 16型L1基因的变异 ,并预测L1蛋白的功能变化。方法 从 19份中国新疆南部地区维吾尔族妇女宫颈癌活检组织标本中提取DNA ,以此DNA为模板 ,PCR扩增HPV16L1全长基因 ,PCR产物直接测序或克隆后测序 ,分析新疆维吾尔族妇女宫颈癌组织HPV16L1基因多态性及HPV16L1蛋白功能的变化。结果PCR检测结果显示宫颈癌组织中HPV16L1阳性率为 84 2 1% (16 / 19) ;测序和序列分析表明L1基因核苷酸多处发生变异 ,并引起编码氨基酸的变异 ,L1基因在核苷酸水平形成上 6种突变模式(XJL1 1～XJL1 6 ) ,各模式与HPV16原型比较 ,同源性在 99 6 9%～ 99 87%之间。XJL1 1发生的 4处变异是所有变异序列所共有的 ,XJL1 2～XJL1 6在XJL1 1变异的基础上增加了不同的变异 ;在氨基酸水平上形成 4种突变模式 ,其中XJL1 1/ 2 / 3突变模式占 76 92 % (8/ 13) ,是突变的主流模式 ;以上突变引起HPV16L1蛋白疏水性和抗原性的改变 ,继而改变了L1蛋白的结构及功能。结论 中国新疆南部地区维吾尔族妇女宫颈癌患者组织中HPV16L1基因发生多位点变异 ,并形成多种突变模式和突变的主流模式 ;这些突变引起HPV16L1蛋白疏水性和抗原性的改变 ,提示HPV16L1基因突变可能与HPV16
Objective To investigate the mutation of human papillomavirus type 16 L1 gene in Uigur women from southern Xinjiang and to predict the function of L1 protein. Methods DNA was extracted from 19 specimens of cervical cancer from Uyghur women in southern Xinjiang, China. The DNA was used as a template to amplify the full-length HPV16 L1 gene. The PCR products were directly sequenced or sequenced. The prevalence of cervical cancer in Uighur women Tissue HPV16L1 gene polymorphism and HPV16L1 protein function changes. Results The positive rate of HPV16L1 in cervical cancer tissues was 84 2 1% (16/19) by PCR. Sequencing and sequence analysis showed that there were multiple nucleotide variations in L1 gene and resulted in the variation of the encoded amino acids. There were 6 mutation patterns (XJL1 1 ~ XJL1 6) on the acid level, and the homology was 99 6 9% ~ 99 87% in comparison with the HPV16 prototype. XJL1 2 ~ XJL1 6 added different mutations on the basis of XJL1 1 mutation; 4 mutation patterns were formed at the amino acid level, of which XJL1 1/2/3 The mutation pattern accounted for 76.92% (8/13), which was the main mode of mutation. The above mutation caused the change of hydrophobicity and antigenicity of HPV16L1 protein, which in turn changed the structure and function of L1 protein. Conclusion HPV16L1 gene in cervical cancer patients from southern Uygur in Xinjiang in China has multi-locus mutation and forms a variety of mutation patterns and mutations in the mainstream model. These mutations cause changes in the hydrophobicity and antigenicity of HPV16 L1 protein, suggesting that HPV16 L1 gene mutation Probably with HPV16