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目的探讨喉癌细胞ｐ５３基因突变与人乳头状瘤病毒（ＨＰＶ）感染的关系及其临床意义。方法应用星普旋转离心柱Ｂ溶液系统，结合聚合酶反应链多态性分析（ＰＣＲ－ＳＳＣＰ）及ＤＮＡ序列分析（ＰＣＲ－ＤＮＡＳｅｑｕｅｎｃｉｎｇ），对３６例喉癌组织新鲜标本进行ＨＰＶ定型、相对定量及ｐ５３基因第４至第８外显子突变检测。结果３６例喉癌标本中有１３例发生基因点突变或转码突变，其中１０例为ＨＰＶ１６／１８感染者，且与细胞内病毒含量呈正相关。２４例晚期喉癌（临床Ⅲ－Ⅳ期）有颈淋巴结转移者突变检出率明显高于无转移者（Ｐ＜０．０５）。结论喉癌ｐ５３基因突变与ＨＰＶ１６／１８感染有关，晚期喉癌颈淋巴结转移与ｐ５３基因突变有关。
Objective To investigate the relationship between p53 gene mutation in human laryngeal carcinoma and human papillomavirus (HPV) infection and its clinical significance. Methods PCR-SSCP and PCR-DNA sequencing were used to detect the HPV types of 36 specimens of laryngeal carcinoma and their relative quantification Detection of p53 mutations in exon 4 to exon 8. Results Thirteen of 36 specimens of laryngeal cancer had gene mutation or transcoding mutations, of which 10 were HPV16 / 18 infected and had positive correlation with the intracellular viral content. The detection rate of mutation in 24 cases of advanced laryngeal carcinoma (stage Ⅲ-Ⅳ) with cervical lymph node metastasis was significantly higher than that without metastasis (P <0.05). Conclusion The mutation of p53 gene in laryngeal carcinoma is related to HPV16 / 18 infection. The metastasis of cervical lymph node in advanced laryngeal carcinoma is related to the mutation of p53 gene.