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为解决杂菌污染导致绝缘油油质劣化的问题, 对分离杂菌进行鉴定, 考察培养条件及其对介质损耗、pH、微水含量和表面张力的影响, 从而提出解决方法.采用唯一碳源培养基分离杂菌并借助生化试验、16sDNA技术鉴定种属.通过吸光度的变化探讨杂菌适宜生存温度并在此条件下对上述4种指标进行70 d的检测.结果表明:成功分离1种芽孢杆菌, 在绝缘油中最适生长温度为37℃.该菌能够导致绝缘油介质损耗变大且导致油质环境酸化.绝缘材料的使用能够维持介质损耗在合格范围内, 并一定程度抑制杂菌生长.绝缘油的表面张力变化与杂菌和绝缘材料不存在明显关系.芽孢杆菌可在绝缘油中存活且最适生长温度为37℃, 并导致油质劣化.“,”To solve the problem of insulating oil quality deterioration caused by bacterial contamination, we indentified the infectious microbes existing in the inferior insulating oil and confirmed the optimum growth temperature. Essentially, based on the effects of bacteria on dielectric loss, pH, moisture content and surface tension, we put forward some solutions to this problem. Solo carbon source was performed to screen out the infectious microbes and its species were indentified by biochemical experiments combining with 16 sDNA technology. The optimum growth temperature was confirmed based on the results of absorbance value. Meanwhile, a 70-day electrical test was designed to check the function of bacteria on four indexes of insulating oil. Bacillus was successfully indentified and the optimum growth temperature was 37 ℃ in insulating oil. Both the increase of dielectric loss and acidification of oil environment were determined in insulating oil infected by bacillus. However, the insulating media were employed to keep the dielectric loss in a standard range and partly inhibit the growth of bacillus. Besides, surface tension and bacillus or insulating media did not show obvious relations in the experiment. Insulating oil can be infected by bacillus and the optimum temperature of growth is 37 ℃, leading to the deterioration of oil.