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以‘洛阳红’牡丹(Paeonia suffruticosa‘Luoyanghong’)切花为试材,研究10μL·L-1乙烯处理、1μL·L-1乙烯抑制剂1-MCP处理、90 g·L-1葡萄糖处理、90 g·L-1葡萄糖+10μL·L-1乙烯复合处理对切花花色和花青素苷含量的影响,以蒸馏水处理为对照。研究结果表明:乙烯处理的切花花色明度增加,红度和彩度下降,花瓣花青素苷含量下降;1-MCP处理与对照无显著差异;葡萄糖处理花色明度下降,红度和彩度增加,花瓣花青素苷含量增加;葡萄糖乙烯复合处理对切花花色及花青素苷积累也有明显改善与促进作用。对花青素苷合成相关基因表达量分析的结果表明,乙烯处理对基因表达有抑制作用,而葡萄糖处理有显著的正调控作用,葡萄糖乙烯复合处理则显著缓解了单独乙烯处理对基因表达的负调控作用。葡萄糖信号和乙烯信号之间一定程度上存在互作,葡萄糖缓解了乙烯对牡丹切花花青素苷合成的抑制作用。
The cuttings of Paeonia suffruticosa’Luoyanghong ’were used to study the effects of 10μL·L-1 ethylene treatment, 1μL·L-1 ethylene inhibitor 1-MCP treatment, 90g · L-1 glucose treatment, 90 g · L-1 glucose + 10μL · L-1 ethylene on the cut flower color and anthocyanin content, with distilled water as a control. The results showed that the lightness and chroma of cut flowers decreased, while the content of anthocyanin in petals decreased. The treatment with 1-MCP had no significant difference with the control. The lightness and the saturation of glucose decreased, Petal anthocyanin content increased; Glucose and ethylene compound treatment of cut flower color and anthocyanin accumulation also significantly improved and promoted. The results of anthocyanin biosynthesis-related gene expression analysis showed that ethylene treatment inhibited gene expression, while glucose treatment had a significant positive regulatory effect, glucose ethylene composite treatment significantly alleviate the effect of ethylene alone on gene expression negative Regulation. There was interaction between glucose signal and ethylene signal to a certain extent. Glucose alleviated the inhibitory effect of ethylene on anthocyanin synthesis of cut peony flower.