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实验中所选用的DLA5、LB7、HG9细菌来自辽河油田、塔里木油田和江汉油田的油水样。用筛选培养出的3种细菌对青海七个泉油田的原油进行了处理,对毛细管等速电泳、气相色谱、红外光谱实测结果的分析表明,这3种细菌可降解青海七个泉油田原油的长链烷烃、芳烃支链,但对芳环骨架没有影响。代谢过程中产生有机酸、酯、酮类和气体等物质。有机酸主要是乙酸,气体主要是氮气。对微生物作用前后的原油物性进行测定,结果表明,微生物作用后可使原油粘度降低、蜡含量下降、凝固点降低。提高采收率是一种多因素协同作用的结果。
The DLA5, LB7 and HG9 bacteria used in the experiment come from oil and water samples of Liaohe Oilfield, Tarim Oilfield and Jianghan Oilfield. Three kinds of bacteria were screened to process the crude oil of Qijuquan Oilfield in Qinghai Province. Analysis of the results of capillary isokinetic electrophoresis, gas chromatography and infrared spectroscopy showed that these three kinds of bacteria could degrade the crude oil of Qizuquan Oilfield in Qinghai Long-chain alkanes, aromatic branched, but no effect on the aromatic ring skeleton. Metabolic processes produce organic acids, esters, ketones and gases. Organic acids are mainly acetic acid, the gas is mainly nitrogen. The physical properties of crude oil before and after the action of microorganism were measured. The results showed that after the action of microorganism, the viscosity of crude oil decreased, the wax content decreased and the freezing point decreased. Increasing oil recovery is a multifactorial synergistic result.