用三步纯化法从人M_3型白血病细胞中分离纯化出人类肿瘤癌性促凝物(CP)。促凝活性回收率为24％,CP纯化倍数为2481倍。纯化CP在SDS-PAGE上为单一区带,其理化和酶学特性类似于动物肿瘤CP,分子量约为70 000,PI为4.8,在FVⅡ缺乏血浆中以及在含有组织因子(TF)抑制剂情况下仍能激活FX。CP促凝活性能被半胱氨酸蛋白酶抑制剂HgCl_2抑制,纯化CP能与抗动物肿瘤CP抗体形成免疫沉淀反应。 Human tumor oncosis (CP) was isolated and purified from human M_3 leukemia cells by three-step purification. Procoagulant activity recovery was 24%, CP purification fold was 2481 times. Purified CP is a single band on SDS-PAGE and has similar physico-chemical and enzymatic properties to animal tumor CP with a molecular weight of about 70,000 and a PI of 4.8 in the absence of plasma FVII and in the presence of inhibitors of tissue factor (TF) FX can still be activated. CP procoagulant activity can be inhibited by the cystatin HgCl2, and purified CP can form an immunoprecipitation reaction with anti-tumor CP antibodies.