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目的 检测血清中乙型肝炎病毒 DNA拷贝数 ,并了解 HBV感染的不同血清学指标组合相应的 HBV- DNA含量分布 ,以指导临床 .方法 采用 Ampli Sensor PCR定量方法 ,检测 2 0 8份不同临床类型血清标本的 HBV- DNA含量 ,再用EL ISA方法测定 HBV- M,统计不同免疫指标组合的 HBV-DNA平均含量 .结果 病毒量分为高、中、低三度 ,大于 10 7拷贝· m L- 1 为高滴度 ;10 7～ 10 5 拷贝· m L- 1 为中等滴度 ;10 5拷贝· m L- 1 以下为低滴度 .6 0例 HBs Ag(+) HBe Ag(+)HBc Ab(+)血清 ,HBV- DNA全部阳性 ,平均含量为 1.8× 10 8拷贝· m L- 1 ;48例 HBs Ag(+) HBe Ab(+) HBc Ab(+)血清 ,HBV- DNA平均含量为 6 .4× 10 6 拷贝· m L- 1 ;30例 HB-s Ag(+) HBc Ab(+)血清 ,HBV- DNA平均含量为 8.5× 10 5拷贝· m L- 1 ;13例 HBs Ab(+) HBe Ab (+) HBc Ab(+)血清 ,HBV- DNA平均含量为 2 .1× 10 5拷贝· m L- 1 .结论 定量 PCR可真实反应 HBV感染、复制及病程变化 ,对乙型肝炎临床诊断及治疗均有较大的指导意义
Objective To detect the copy number of hepatitis B virus DNA in serum and to understand the distribution of HBV DNA in different serological markers of HBV infection in order to guide the clinical practice.Methods Ampli quantitative PCR was used to detect 288 different clinical types HBV-DNA levels in serum samples were measured, and HBV-M was measured by ELISA.Results The viral load was divided into high, medium and low third degree, more than 10 7 copies · m L - 1 for high titers; 10 7 to 10 5 copies · m L -1 for moderate titers; and 10 5 copies · m L -1 for low titers.60 HBs Ag (+) HBe Ag (+) HBcAb (+) serum and HBV-DNA were all positive with the average content of 1.8 × 10 8 copies · m L -1; 48 cases of HBs Ag (+) HBe Ab (+) HBc Ab The content of HBV DNA in serum was 6.4 × 10 6 copies · mL -1 and the average serum HBV-DNA was 8.5 × 10 5 copies · mL -1 in 30 cases of HBsAg (+) HBc Ab (+). Thirteen HBs Ab (+) HBcAb (+) serum, the average content of HBV DNA was 2.1 × 10 5 copies · m L -1 .Conclusion Quantitative PCR can truly reflect the HBV infection, replication and course of disease, Hepatitis B Pro Bed diagnosis and treatment have a greater guiding significance