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目的研究姜黄素对砷致大鼠睾丸生殖细胞凋亡的拮抗作用。方法将50只健康清洁级Wistar雄性大鼠随机分为5组,分别为对照(双蒸水)组和亚砷酸钠(100 mg/L)染毒组及低、中、高剂量姜黄素(100 mg/L亚砷酸钠+25、50、100mg/kg姜黄素)干预组,每组10只。亚砷酸钠采用自由饮用方式进行染毒;姜黄素采用经口灌胃方式染毒,每周3次,连续8周。测量生精小管平均直径;测定大鼠血清睾酮浓度及睾丸生精细胞凋亡指数。结果与对照组比较,亚砷酸钠染毒组大鼠血清睾酮的水平降低,生精小管直径缩小,而睾丸生精细胞凋亡指数升高,差异有统计学意义(P<0.01)。与亚砷酸钠染毒组比较,中、高剂量姜黄素干预组大鼠血清睾酮的水平升高,生精小管直径增大,而睾丸生精细胞凋亡指数下降,差异均有统计学意义(P<0.05)。且随着姜黄素染毒剂量的升高,大鼠血清睾酮水平和生精小管直径均呈上升趋势,而睾丸生精细胞凋亡指数呈下降趋势。结论姜黄素对砷致大鼠睾丸生殖细胞凋亡具有拮抗作用。
Objective To study the antagonistic effect of curcumin on germ cell apoptosis induced by arsenic in rats. Methods Fifty healthy male Wistar rats were randomly divided into five groups: control (double distilled water) group and sodium arsenite (100 mg / L) and low, medium and high dose of curcumin 100 mg / L sodium arsenite + 25, 50, 100 mg / kg curcumin) intervention group, 10 rats in each group. Sodium arsenite was exposed to free drinking; curcumin was orally administered orally 3 times a week for 8 weeks. The mean diameter of seminiferous tubules was measured. The serum testosterone concentration and apoptosis index of testicular spermatogenic cells were measured. Results Compared with the control group, the serum testosterone level decreased, the diameter of seminiferous tubules decreased, and the apoptosis index of testicular spermatogenic cells increased in the arsenite-treated group. The difference was statistically significant (P <0.01). Compared with the sodium arsenite treatment group, the levels of serum testosterone increased, the diameter of the seminiferous tubules increased, and the apoptosis index of spermatogenic cells decreased in the medium and high dose curcumin intervention groups, the differences were statistically significant (P <0.05). And with the dose of curcumin increased, the level of serum testosterone and the diameter of seminiferous tubules showed an upward trend, while the apoptosis index of testicular spermatogenic cells showed a downward trend. Conclusion Curcumin has an antagonistic effect on the germ cell apoptosis induced by arsenic in rats.