论文部分内容阅读
目的:采用高效液相色谱法,比较同地同株凤丹皮中不同部位的丹皮酚和芍药苷含量差异。方法:采用Waters VP-ODS-C18色谱柱(4.6 mm×150 mm,5μm);丹皮酚含量测定流动相甲醇-水(55∶45),检测波长274 nm,柱温35℃,流速1.0mL·min-1;芍药苷含量测定流动相乙腈-0.1%H3PO4(20∶80),检测波长230 nm,柱温35℃,流速1.0 mL·min-1。结果:丹皮酚在1.073~5.365μg,芍药苷在0.593~2.965μg均呈良好线性关系,相关系数分别为0.999 9,0.999 1,平均回收率丹皮酚为99.96%(RSD 0.72%),芍药苷为99.03%(RSD 0.49%)。结论:在选定的同株凤丹皮最细部位的丹皮酚和芍药苷含量均高于最粗部位及中等部位。
OBJECTIVE: To compare the differences of paeonol and paeoniflorin in different parts of the same plant of the same species with high performance liquid chromatography. METHODS: Waters VP-ODS-C18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase consisted of methanol and water (55:45) with a detection wavelength of 274 nm and a column temperature of 35 ℃ · Min-1. The mobile phase was acetonitrile-0.1% H3PO4 (20:80). The detection wavelength was 230 nm. The column temperature was 35 ℃ and the flow rate was 1.0 mL · min-1. Results: Paeonol had a good linearity at 1.073-5.365μg and paeoniflorin at 0.593-2.965μg with correlation coefficients of 0.999 9 and 0.999 1, respectively. The average recoveries of paeonol were 99.96% (RSD 0.72%), Glycoside 99.03% (RSD 0.49%). Conclusion: The contents of paeonol and paeoniflorin in the thinnest part of the same plant were higher than those in the coarsest part and middle part.